PCOS Treatment Report

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Serum β-Klotho concentrations are increased in women with polycystic ovary syndrome

https://www.sciencedirect.com/science/article/abs/pii/S1043466620302040?via%3Dihub

https://www.ncbi.nlm.nih.gov/pubmed/32673996?dopt=Abstract

Serum β-Klotho concentrations are increased in women with polycystic ovary syndrome.

Cytokine. 2020 Jul 13;134:155188

Authors: Bednarska S, Fryczak J, Siejka A

Abstract

Polycystic ovary syndrome (PCOS) is a significant cause of menstrual disorders and infertility in women. The aim of this study was to evaluate the levels of Klotho expression in women with polycystic ovary syndrome. The study involved 67 patients with PCOS, as well as 18 controls. Serum levels of FGF19, FGF21 and β-Klotho were measured by ELISA technique. The blood samples were obtained between days 2 and 7 of menstrual cycle. The concentrations of β-Klotho, FGF19 and FGF21 were significantly increased in patients with PCOS; and appear to be statistically significant predictors of PCOS incidence. FGF19 (p = 0.031, OR = 1.01), exact β-Klotho levels (p = 0.022, OR = 1.01) and β-Klotho beyond the reference range (p = 0.008, OR = 4.66) were the most strongly correlated with the diagnosis of PCOS. In conclusion, FGF19, FGF21 and β-Klotho are increased in PCOS, and elevated β-Klotho concentration may become an useful diagnostic test for this disease. However, additional studies are required to further substantiate this observation.

PMID: 32673996 [PubMed – as supplied by publisher]

Methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms in the risk of polycystic ovary syndrome

https://portlandpress.com/bioscirep/article/doi/10.1042/BSR20200995/225735/Methylenetetrahydrofolate-reductase-gene

https://watermark.silverchair.com/bsr-2020-0995.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAABJgwggSUBgkqhkiG9w0BBwagggSFMIIEgQIBADCCBHoGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM11Airg2PZo1kF5IYAgEQgIIESyTQOK5UkBFpeIMU3inOQlO5L4tx9Nqd4uGmS9rhzSb0lNYR4mpWjnYSSnaAH_CojMSq_jZSqYHrZVIrottT-jMf14goMOXPAxgmqMvvPNqpLn4n-y2av-p6KA__tz_-gQoycolAeE-bU0Zh19wuOq9VdEiCOMEE_Zuw6hx3wjSUN7m7KjDFGhJXjNcw3nyInomQPfQhQnbGtheFj8bSfobCg8iEri0IdzpwFmU87-zf5Fm4wU95X3jaobAEVIB7VxPLFjmm9NogPTWhJcRidrGPGIhyBgiN6Tup7_cIdsbr9l63OVNablDgnudVtuUOvH4zosRafSoa0jMiBR28ZGDUUMdNjQjmLMSnOmeQ97nzqrsYdxO3dOwWeigl8F2FNrQL8AsbiyKWZ6Wrtyo3Q2uU3bUgnLtcTo3wRGi9uRqQ5LtvIb9LzoIKPau28r9oJ9tivzRoaIjbXhB-jOYsH7adyR8wwOHF1baTrXEgykTYrs89sXuy3g_SubW_A3yXQDe4e_xiAuy_aukGWsWu3pPKsWLBB-dgD5HN5vIgec-ehw90toq5UznNpbvWjJvEXLu92lnHW6EuC6tVoNXYFNcW1jb1WU8nzYjsJNtRk6rHNDg-JhykDJw1MNMuraAJG_2vCr9740OoKonrS0sGJHtWMKgM8-qa47SqrJxiaoNSzGScRyFudI4jQOY8gX_EoRxNQAmiJUFX_pIdArOlzUWWY-fc7GUg7CEdAg8v1GPEepLqH4h7sHFwCNw_Yaweh1EW8yqQ6ld99ms25A2NkEMU510ODd-Cp3kLrg3vAGK-u4UmIJpvVYC4eNXrrGZnajijRRcuiGEvuJwBvy0cXHqiE8RKIEB6fNEQKJioEWIGWVQ2IUXbdD5Wohu2KQM5Z_BpBqT0uzmc0OopJ7omikHVxuPuwNEKw9U3c-YE4pKx2L9G61WqHleopwJFOn1iSfsDnsItxor9zOfspAySfqp6wKEKFANoG0fhP2-xqlSgkeO0ZPKRXAGByJYEfGoXyrW3cm-TaiUF5TA-RLNjEKqWIyuAtbvfnnzxj1F6HBCIi6vl9577ZC8J6eZceEnDs3IF-w9vZK9o00F8uqTjGwljmOkiq_geKF3plyYEhpo0LUwMC_OTVXgVpdFKermkuCR1DgLctNJ6GebR7y4PDB3DhhlROxw_T9pgFxHbXEWKZC7xZ6bVw8m4rciMusCgPUWokuct–QT2lqCvSDhmjOzBsGgjW4uAJl1loQ-xzrCQa_KW3TRmgA5HvVXEboIRqAnBbw3OlbHhGHna98SzSQkpX7URPZndQXIqyV336w5BYFLaVdtDv-CcrPJkwlNBrphU-mjUEaYwbHjog1eLYInqrZnszQmLnhBuMQzWJI8VsNuOPdGLknqQSrx-o9O9pg0p_JPztinQINcJprrH4z24JlZdS0P9SYoCHNb5rqJQoSXQgdlWlY29tQ

https://www.ncbi.nlm.nih.gov/pubmed/32639550?dopt=Abstract

Methylenetetrahydrofolate reductase gene polymorphisms in the risk of polycystic ovary syndrome and ovarian cancer.

Biosci Rep. 2020 Jul 08;:

Authors: Xiong Y, Bian C, Lin X, Wang X, Xu K, Zhao X

Abstract

Polymorphisms of methylenetetrahydrofolate reductase (MTHFR) in hormone metabolism pathways might cause metabolic disturbances and contribute to the development of polycystic ovary syndrome (PCOS) and ovarian cancer, but the published studies were inconsistent. The aim of this study was to evaluate the MTHFR C677T (rs1801133) and A1298C (rs1801131) gene polymorphisms in the risk of PCOS and ovarian cancer by meta-analysis. A comprehensive electronic search was conducted in databases for studies published from 1995 to 2020. The pooled ORs were calculated by Revman 5.2 software. Twenty-nine articles including 45 case-control studies were included. We found that MTHFR C677T polymorphisms were correlated with elevated PCOS risk (TT vs. CT+CC: OR=1.41, 95%CI=1.20-1.67; TT+CT vs. CC: OR=1.54, 95%CI=1.07-2.22; CT vs. CC+TT: OR=1.18, 95%CI-1.04-1.33; TT vs. CC: OR=1.47, 95%CI=1.03-2.11; T vs. C: OR=1.25, 95%CI=1.06-1.47), which were more obvious in Middle Eastern subgroup. MTHFR A1298C polymorphisms were also associated with overall PCOS susceptibility (CC vs. AC+AA: OR=2.55, 95%=1.61-4.03; CC+AC vs. AA: OR=1.84, 95%CI=1.04-3.28; CC vs. AA: OR=2.66, 95%CI=1.68-4.22; C vs. A: OR=1.67, 95%CI=1.03-2.71), which were mainly reflected in Asian subjects. For ovarian cancer, MTHFR C677T polymorphisms were only related with elevated ovarian cancer risk in Asian population, while no significant association was found for A1298C polymorphisms. This meta-analysis suggested that MTHFR C677T and MTHFR A1298C polymorphisms were correlated with elevated PCOS risk. MTHFR C667T only posed a higher risk for ovarian cancer in Asians instead of other populations, while MTHFR A1298C polymorphisms were not related to ovarian cancer risk. Further studies are needed to validate the conclusion.

PMID: 32639550 [PubMed – as supplied by publisher]

Distinct subtypes of polycystic ovary syndrome with novel genetic associations

https://journals.plos.org/plosmedicine/article?id=10.1371/journal.pmed.1003132

Distinct subtypes of polycystic ovary syndrome with novel genetic associations: An unsupervised, phenotypic clustering analysis
Matthew Dapas,Frederick T. J. Lin,Girish N. Nadkarni,Ryan Sisk,Richard S. Legro,Margrit Urbanek,M. Geoffrey Hayes ,Andrea Dunaif
Published: June 23, 2020https://doi.org/10.1371/journal.pmed.1003132

Epigenetic marks in Polycystic Ovary Syndrome

https://www.ncbi.nlm.nih.gov/pubmed/31580245?dopt=Abstract

http://www.eurekaselect.com/175312/article

Epigenetic marks in Polycystic Ovary Syndrome.

Curr Med Chem. 2019 Oct 03;:

Authors: Motta AB

Abstract

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that affects women in their reproductive age. Recent studies have shown that genes have an important role in the etiology of PCOS. However, the precise way in which these genes are transcriptionally and post-transcriptionally regulated is poorly understood. The aim of the present review is to provide updated information on miRNAs and DNA methylation as epigenetic marks of PCOS. The data presented here allow concluding that both microRNAs and DNA methylation can be considered as possible useful biomarkers when choosing the treatment for a specific PCOS phenotype and thus represent two important tools for the diagnosis and treatment of PCOS patients.

PMID: 31580245 [PubMed – as supplied by publisher]

Colocalization of Polycystic Ovary Syndrome Candidate Gene Products in Theca Cells Suggests Novel Signaling Pathways [DENND1A]

https://academic.oup.com/jes/article/3/12/2204/5569849

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839531/

Colocalization of Polycystic Ovary Syndrome Candidate Gene Products in Theca Cells Suggests Novel Signaling Pathways
Rewa Kulkarni, Maria E Teves, Angela X Han, Jan M McAllister, Jerome F Strauss, III Author Notes
Journal of the Endocrine Society, Volume 3, Issue 12, December 2019, Pages 2204–2223, https://doi.org/10.1210/js.2019-00169
Published: 16 September 2019 Article history
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Abstract
Genome-wide association studies identified loci associated with polycystic ovary syndrome (PCOS), including those near the LH receptor gene (LHCGR), a clathrin-binding protein (DENND1A) that functions as a guanine nucleotide exchange factor, and the gene encoding RAB5B, a GTPase involved in vesicular trafficking. We proposed that these three PCOS loci could be assembled into a functional network that contributes to altered gene expression in theca cells, resulting in increased androgen synthesis. The functional significance of this network was supported by our discovery that a truncated protein splice variant of the DENND1A gene, termed DENND1A.V2, is elevated in PCOS theca cells, and that forced expression of DENND1A.V2 in normal theca cells increased CYP11A1 and CYP17A1 expression and androgen synthesis, a hallmark of PCOS. In this study, we demonstrate the colocalization of LHCGR, DENND1AV.2, and RAB5B proteins in various cellular compartments in normal and PCOS theca cells by immunofluorescence. Human chorionic gonadotropin and forskolin stimulation was shown to affect the cytoplasmic distribution of LHCGR, DENND1A.V2, and RAB5B. DENND1A.V2 accumulated in the nuclei of the theca cells. Moreover, PCOS theca cells, following forskolin treatment, had a significantly greater relative abundance of nuclear DENND1A.V2. RAB5B also accumulated in the nuclei of PCOS theca cells treated with forskolin. In contrast, LHCGR did not enter the nucleus. This cytological evidence, and the previously reported increase in androgen biosynthesis with forced expression of DENND1A.V2 in normal theca cells, raises the possibility that DENND1A.V2 and RAB5B participate in increasing transcription of genes involved in androgen synthesis.

High throughput circRNAs sequencing profile of follicle fluid exosomes of polycystic ovary syndrome patients

The obtained results provide a valuable addition to the published studies on the mechanism of PCOS pathogenesis by revealing a wide variety of new circRNAs, miRNA, and gene targets that merit further investigation.

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.28201

https://www.ncbi.nlm.nih.gov/pubmed/30779115?dopt=Abstract [Read more…] about High throughput circRNAs sequencing profile of follicle fluid exosomes of polycystic ovary syndrome patients

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